While neb develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications. Do you need advice choosing the right reaction buffer for your double digests. The quickload purple low molecular weight dna ladder is not intended for precisequantification of dna mass but can be used for approximating the mass ofdna in comparably intense samples of similar size. Rapid restriction enzymefree cloning of pcr products. Compare quickload 100 bp dna ladder from new england biolabs on. Quick load purple 50 bp dna ladder is a premixed, readyto load molecular weight marker containing one tracking dye. Aw dynamometer high quality dynamometers built 100% inhouse. Quickload 100 bp dna ladder is a readytoload molecular weight marker containing bromophenol blue.
Browse biorads assortment of easytouse nucleic acid electrophoresis standards. Quickload purple 100 bp dna ladder is a premixed, readytoload molecular weight dna marker containing one tracking dye. New england biolabs uk ltd tridye 100 bp dna ladder. It consists of purified and ligated blunt ended basic unit repeats of 100 bp.
We have numerous interactive online tools for these and other questions in your daily lab work. Quickload 100 bp dna ladder new england biolabs japan. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp. The nebnext multiplex small rna library prep set for illumina. Convenient, readyto load format with neb s proprietary purple loading dye size range.
Quickload purple 100 bp dna ladder is stable for at least 6 months at 25c. It is step ladder with 100 bp differences between the dna fragments. By selecting the generuler dna ladder of interest in table 1, you will be directed to the corresponding product page for more details and ordering information. Pcr products were run on a 1% agarose gel at 85v for 1 hour with a 100 bp ladder. In order to ensure quality for your customers, you need accurate testing equipment that your team can easily operate. Quickload purple 100 bp dna ladder n0551 new england biolabs. Choose a dna, rna, genome editing, qpcr calculator from neb, a leader in production and supply of reagents for the life science industry.
Quickload purple 50 bp dna ladder is a premixed, readyto load molecular weight marker containing one tracking dye. A novel multiplex pcrrflp method for simultaneous genotyping. Onetaq quickload 2x master mix with standard buffer is an optimized, readytouse blend of taq and deep vent dna polymerases ideally suited to routine pcr applications from a variety of templates, including pure dna solutions, bacterial colonies and cdna products. Rapid cloning into any vector with no additional sequence added. Quickload purple 2log dna ladder from new england biolabs. If samples need to be diluted, use te or other buffer of minimal ionic strength. There have been no changes to product formulation or specifications.
The following file naming structure is used to name the majority of these document files. Try the quickload 1 kb plus dna ladder from neb, a premixed, readytoload molecular weight marker ranging from 100 bp to 10 kb. Neb s quickload purple dna ladders offer sharp, uniform bands with easily identifiable reference bands. The digested dna includes fragments ranging from 100 bp to 10 kb. Or do you plan a complex dna assembly and you need a tool for your primer design. A single convenient ladder for 100 bp up to 10 kb 100 bp and 1 kb ladder in one. Despite a stay at home advisory being put in place in massachusetts, usa, we are deemed an essential business, and our manufacturing and distribution teams continue to be fully operational. This 1kb plus dna ladder, neb was previously named the 2log dna ladder. Nebuilder hifi dna assembly or gibson assembly reactions. Quickload taq 2x master mix should be used at a 1x concentration with dna template and primers in a total reaction volume of 25 or 50. Nucleic acid standards life science research biorad. New england biolabs is working diligently to ensure we keep our employees and their families safe, while maintaining our business continuity. Dna rna markers and ladders new england biolabs gmbh.
The approximate mass of dna in each of the bands is provided assuming a 1. The quick load purple 50 bp dna ladder consists of a proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for. Find more information about nebuilder in the resources tab. Apr 06, 2012 here we introduce a rapid, costeffective method of generating molecular dna probes in just under 15 minutes without the need for expensive, timeconsuming gelextraction steps. The quickload purple 100 bp dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. The quickload purple 50 bp dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. Onetaq quickload 2x master mix with standard buffer. Onetaq quickload 2x master mix with standard buffer, new.
Securetabs is a chain of pharmacies that serve people thru the world wide web. In general, it is recommended to load 1l per mm of gel lane. Neb also offers a quickload version of this ladder with purple dye. Enjoy the convenience of a premixed, readyto load dna ladder. Quickload purple 50 bp dna ladder is stable for at least 6 months at 25c. Use the neb tm calculator to estimate an appropriate annealing temperature when using neb pcr products. As an example, we enzymatically concatenated six variable strands 50 bp. In general, it is recommended to load 1 l per mm of gel lane. Resistance genes on large selftransmissible plasmids.
Onetaq quickload dna polymerase is compatible with usercloning methods and bisulfitetreated dna. Other 100 bp dna ladder this product is available in quick load purple format enjoy the convenience of a readyto load format. Quickload 100bp dna ladder neb inc, massachusetts, usa. What is the fidelity of onetaq quickload dna polymerase. Quick load purple low molecular weight dna ladder is a premixed, readyto load molecular weight marker containing one tracking dye. Multiplex pcr method for minion and illumina sequencing of. The product summary sheet, or data card, includes details for how to use the product, as well as details of its formulation and quality controls. Dna rna markers and ladders neb offers a variety of dna ladders with sizes ranging from 25 bp to 40 kb for use in agarose gel electrophoresis. Quick load 100 bp dna ladder is a premixed, readyto load molecular weight marker containing bromophenol blue as a tracking dye. Thermo scientific orangeruler 100 bp dna ladder 100 1500 bp is designed for precise sizing of pcr products and other doublestranded dna fragments in agarose or nondenaturing polyacrylamide gels. New england biolabs novex sharp prestained protein standard invitrogen serva triple color protein standard serva electrophoresis. Select molecular rulers, ez load standards, and pulsedfield standards. Quickload purple 100 bp dna ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 12 bands. Quickload 100 bp dna ladder, new england biolabs vwr.
Gels were run with appropriate size ladders noted in each specific section which included a quickload purple 100 bp dna ladder neb, cat no. Herein, we describe a novel cloning strategy for pcramplified dna which employs the type iis restriction endonuclease bsai to create a linearized vector with four baselong 5. Quickload taq 2x master mix is also stable for one week at 4c, so for daily use, an aliquot may be kept at 4c. Quickload purple dna ladders from neb are premixed, readytoload molecular weight markers. Quickload 100 bp dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dye. Quickload 100 bp dna ladder is a premixed, readytoload molecular weight marker containing bromophenol blue as a tracking dyemolecularweight size markers are a set of standards used to identify the approximate size of a molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel matrix. The comb was removed once the gel had been allowed to completely set. Neb, quickload purple 100 bp dna ladder proficient lab. Quickload purple 100 bp dna ladder new england biolabs. N0551s, quickload purple 1 kb dna ladder neb, cat no. Neb also offers a wide range of conventional doublestranded dna molecular weight markers.
Quick load low molecular weight dna ladder has been discontinued and replaced with n0557 quick load purple low molecular weight dna ladder. A chain of online dispensaries means there are different online pharmacies that offer the same products at the same price. A rapid, costeffective method of assembly and purification. Protocol for quickload 100 bp dna ladder n0467 neb. Onetaq quickload 2x master mix with standard buffer 100 rxns. The quickload dna ladder is not intended for precise quantification of dna mass but can be used for approximating the mass of dna in comparably intense samples of similar size. The primers were designed using primer3 software with primer. The success of your business relies on the quality of your products and services.
All include a vial of gel loading dye, purple 6x, no sds. Plasmid puc5xrep contains five 32 bp repeats, making it unstable in a. This format is available for the low molecular weight, 50 bp, 100 bp and 1 kb plus dna ladders. N0552s and a quickload 1 kb extend dna ladder neb, cat no. Notably, the insert preparation does not require any restriction enzyme. Quickload purple dna ladder is convenient and in readytoload format with nebs proprietary purple loading dye. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis.
Neb also offers a quick load version of this ladder with purple dye. Quickload 1 kb plus dna ladder new england biolabs. The digested dna includes fragments ranging from 100 bp to 1,517 bp. Reagents supplied the following reagents are supplied with this product. Perfect 50% gc content eliminates spurious migration due to sequence variation, ensuring that the fragments migrate exactly according to their specified size. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular.
Readings between 160110 and 180110 usually indicate stage 2 hypertension, which puts you at high risk for lifethreatening problems such as heart attack and stroke. Onetaq quick load 2x master mix with standard buffer is an optimized, readytouse blend of taq and deep ventr tm dna polymerases ideally suited to routine pcr applications from a variety of templates, including pure dna solutions, bacterial colonies and cdna products. Onetaq quick load 2x master mix with standard buffer 500 rxns. The dna ladder consists of a proprietary plasmid which is digested to completion with appropriate restriction enzymes to yield 11 bands suitable for use as molecular weight. Quick load purple 2log dna ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis. The quickload purple 50 bp dna ladder consists of a proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 17 bands suitable for. Generuler dna ladders are ideal for dna sizing and approximate quantification of a wide range of doublestranded dna ranging from 10 bp. Quickload taq 2x master mix is stable for fifteen freezethaw cycles when stored at 20c. In silico identification of novel biomarkers and development. Quickload 100 bp dna ladder from new england biolabs. The key to accurate band analysis is to use the correct dna ladder for your particular application. This digested dna includes fragments ranging from 100 bp to 10 kb. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 19 bands suitable for use as molecular weight standards for agarose gel electrophoresis.
Quickly determine the size of your dna, using evenlyspaced bands and easilyidentifiable reference bands. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2000 bp. Quickload purple 100 bp dna ladder consists of proprietary plasmids, which are digested to completion with appropriate restriction enzymes to yield 12 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Due to the covid19 pandemic, precautions have been taken at our offices around the world to ensure the safety of our staff and to interrupt the infection chains as far as possible.
If not well mixed, the load may float out of the wells. Jul 16, 2019 a 100 bp dna ladder new england biolabs was used as the molecular weight marker mwm. Quickload purple low molecular weight dna ladder neb. Quick load 1 kb plus dna ladder from neb is a premixed, readyto load molecular weight marker ranging from 100 bp to 10 kb.
New england biolabs uk ltd quickload taq 2x master mix. The dna ladder consists of proprietary plasmids which are digested to completion with appropriate restriction enzymes to yield 10 bands suitable for use as molecular weight standards for agarose gel electrophoresis. Convenient, readytoload format with nebs proprietary purple loading dye. Nebs popular 2log dna ladder is now available in a quick load. Download a pdf containing pricing for our full product list. While neb develops and validates its products for various applications, the use of this product. Tracking dye does not leave a shadow under uv light. The following reagents are supplied with this product. Use our molecular ruler to accurately assign molecular weights to dna fragments separated by gel electrophoresis. Detailed information on features is also available in the help file.
1367 1586 225 711 686 866 1380 730 1310 397 617 297 1447 1559 1049 953 829 1149 928 1665 253 548 1423 800 147 1428 877 110 1132 104 94 1373 855